发明授权
- 专利标题： Method for imaging zinc activation within a mitochondrion using a two-photon fluorescent probe, and method for manufacturing the two-photon fluorescent probe
- 专利标题（中）： 使用双光子荧光探针在线粒体内锌激活成像的方法和用于制造双光子荧光探针的方法
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申请号： US13980946申请日： 2011-10-18
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公开(公告)号： US09006452B2公开(公告)日： 2015-04-14
- 发明人: Hwan Myung Kim
- 申请人： Hwan Myung Kim
- 申请人地址： KR Gyeonggi-do
- 专利权人： AJOU University Industry-Academic Cooperation Foundation
- 当前专利权人： AJOU University Industry-Academic Cooperation Foundation
- 当前专利权人地址： KR Gyeonggi-do
- 代理机构： Frommer Lawrence & Haug LLP
- 代理商 Ronald R. Santucci
- 优先权： KR10-2011-0006521 20110121
- 国际申请： PCT/KR2011/007750 WO 20111018
- 国际公布： WO2012/099317 WO 20120726
- 主分类号： C07F9/6558
- IPC分类号： C07F9/6558 ; C07F9/54 ; G01N33/84 ; G01N33/533 ; C07D417/14
摘要：
Provided is a two-photon fluorescent probe, and more particularly, a two-photon fluorescent probe which is one or more selected from compounds represented by Formulae 1 and 2, a method for manufacturing the same, and an imaging method of zinc ions within the mitochondrion using the same. Since two probes are introduced into one molecule, the two-photon fluorescent probe of the present invention can selectively dye the mitochondria, simultaneously with reacting with zinc ions, thereby generating intense fluorescence. Thus, the two-photon fluorescent probe of the present invention can be used for the imaging of zinc ion distribution and activation within the mitochondrion in living cells or intact biological tissues.