发明授权
- 专利标题: Method for transfecting nucleic acid to cell and nucleic acid complex
- 专利标题(中): 将核酸转染细胞和核酸复合物的方法
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申请号: US13808695申请日: 2011-07-07
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公开(公告)号: US09057067B2公开(公告)日: 2015-06-16
- 发明人: Masayuki Fujii
- 申请人: Masayuki Fujii
- 申请人地址: JP Osaka
- 专利权人: Kinki University
- 当前专利权人: Kinki University
- 当前专利权人地址: JP Osaka
- 代理机构: Wenderoth, Lind & Ponack, L.L.P.
- 优先权: JP2010-157367 20100710
- 国际申请: PCT/JP2011/065612 WO 20110707
- 国际公布: WO2012/008361 WO 20120119
- 主分类号: A61K48/00
- IPC分类号: A61K48/00 ; C07K4/00 ; C12N15/87 ; C12N15/113 ; C12N15/11
摘要:
A method for transfecting nucleic acid to cell comprising a step for forming a nucleic acid complex by bringing a double-stranded nucleic acid molecule into contact with a nucleic acid carrier having an amino acid sequence of alternating a basic amino acid and a hydrophobic amino acid, which has a peptide chain that forms a β-sheet structure in which a side chain of a positively charged basic amino acid is disposed on one surface side and a side chain of a hydrophobic amino acid is disposed on the opposite surface side in the presence of the double-stranded nucleic acid molecule having a double helix structure, and by binding the double-stranded nucleic acid molecule and the peptide chain through either one or both of the electrostatic interaction between the side chains of the basic amino acid and phosphate groups and hydrogen bonds between the double stranded-nucleic acid molecule and the peptide chain and a nucleic acid complex used for the same are disclosed.
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