An amplifier circuit and an operation method thereof are provided. The amplifier circuit includes two stages of amplifiers. When the amplifier circuit is operated in a high gain mode, the two stages of amplifiers are operated normally to provide high gain. When the amplifier circuit is operated in a low gain mode, the second stage of amplifier is turned off, and the first stage of amplifier is coupled to output terminals of the amplifier circuit through signal isolation elements so as to form a single stage of amplifier. Therefore, the amplifier circuit can change the total gain value thereof according to a requirement of gain.