A tape cassette includes a tape ejecting port for ejecting outside the tape with a separator being adhered to one of surfaces thereof, a tape conveying roller arranged at an upstream side of a tape conveying direction at the tape ejecting port, the tape conveying roller conveying roller pulling out and conveying the tape while getting in contact with the separator being adhered to one surface of the tape, a separator guiding portion for guiding the separator peeled off from the tape inwardly at the tape ejecting port along a peripheral surface of the tape conveying roller and a separator-take-up spool arranged so as to fix thereto a front end of the separator that has been guided inwardly along the peripheral surface of the tape conveying roller from the separator guiding portion and to take up the separator.