A device, kit and method for detecting the presence or absence of hookworm antigens. The device, kit and method of the present invention may be used to confirm the presence or absence of hookworm in a fecal sample that may be infected with one or more of roundworm, whipworm, tapeworm and heartworm, and whether or not hookworm ova are present in the sample. Further, the device, kit and method of the present invention may be used to confirm the presence or absence of hookworm in a fecal sample excreted by a canine animal as early as nine days after the animal first becomes infected with hookworm.