Methods for determining a point-of-gaze (POG) of a user in three dimensions are disclosed. In particular embodiments, the methods involve: presenting a three-dimensional scene to both eyes of the user; capturing image data including both eyes of the user; estimating first and second line-of-sight (LOS) vectors in a three-dimensional coordinate system for the user's first and second eyes based on the image data; and determining the POG in the three-dimensional coordinate system using the first and second LOS vectors.