Systems, methods, and devices for white point calibration using subtractive color measurements are provided. Specifically, a white point of a display may be calibrated using subtractive color measurements rather than merely additive color measurements. In one example, a display having red, green, and blue pixels may measure the responses in a subtractive color space (e.g., CMY) rather than additive color space (e.g., RGB). Measurements of the display response using subtractive color space may involve providing image data to two or more color channels at once. Thus, any crosstalk effect between channels may be accounted for, even though the same crosstalk effect might not be apparent using additive color measurements in which only a single channel color channel were measured.