The present invention relates to a fluorescence observation device, a domed base and a fluorescence microscope. The fluorescence observation device includes a dome, a multi-angle light-emitting unit and a controller unit. The dome includes a transparent thermal insulation layer and a heat sink layer. The multi-angle light-emitting unit includes multiple directional narrow light field electro-luminescence devices thermally connected to the heat sink layer and adapted to emit light at different angles to pass through the transparent thermal insulation layer. The narrow light field electro-luminescence devices are selectively activated to emit light by the controller unit, so that the illumination angle of the multi-angle light-emitting unit can be adjusted. The fluorescence observation device can be combined with a base to constitute the domed base. Alternatively, narrow light field electro-luminescence devices are mounted on the base.