Invention Grant
- Patent Title: Methods and compositions for detection and analysis of polynucleotides using light harvesting multichromophores
- Patent Title (中): 使用光收获多色素探测和分析多核苷酸的方法和组合物
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Application No.: US14460245Application Date: 2014-08-14
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Publication No.: US09085799B2Publication Date: 2015-07-21
- Inventor: Guillermo C. Bazan , Brent S. Gaylord , Shu Wang
- Applicant: The Regents of the University of California
- Applicant Address: US CA Oakland
- Assignee: The Regents of the University of California
- Current Assignee: The Regents of the University of California
- Current Assignee Address: US CA Oakland
- Agency: Bozicevic, Field & Francis LLP
- Agent Glenn J. Foulds; Bret E. Field
- Main IPC: C12Q1/68
- IPC: C12Q1/68 ; C07H19/04
Abstract:
Methods, compositions and articles of manufacture for assaying a sample for a target polynucleotide are provided. A sample suspected of containing the target polynucleotide is contacted with a polycationic multichromophore and a sensor polynucleotide complementary to the target polynucleotide. The sensor polynucleotide comprises a signaling chromophore to receive energy from the excited multichromophore and increase emission in the presence of the target polynucleotide. The methods can be used in multiplex form. Kits comprising reagents for performing such methods are also provided.
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