In wireless OFDMA systems, midamble is used to facilitate downlink (DL) channel estimation. Midamble signals are transmitted by a base station via a midamble channel allocated in a DL subframe. In a novel symbol-based midamble channel allocation scheme, a midamble channel is allocated in the first or the last OFDM symbol of multiple resource blocks of the subframe, while the remaining consecutive OFDM symbols are used for data transmission. The symbol-based midamble channel provides good coexistence between midamble signals and pilot signals without inducing additional limitation or complexity. Under a novel midamble channel and sequence arrangement, both code sequence and either time-domain or frequency-domain location degrees-of-freedom are considered such that the required number of midamble sequences is substantially smaller than the number of strong interferences. In addition, different midamble sequences are systematically generated based on a base sequence such that the receiving mobile station does not need to memorize all the different code sequences.