Invention Grant
- Patent Title: High fidelity thermostable ligase and uses thereof
- Patent Title (中): 高保真热稳定连接酶及其用途
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Application No.: US14028151Application Date: 2013-09-16
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Publication No.: US09127269B2Publication Date: 2015-09-08
- Inventor: Francis Barany , Weiguo Cao , Jie Tong
- Applicant: Cornell Research Foundation, Inc.
- Applicant Address: US NY Ithaca
- Assignee: Cornell Research Foundation, Inc.
- Current Assignee: Cornell Research Foundation, Inc.
- Current Assignee Address: US NY Ithaca
- Agency: LeClairRyan, a Professional Corporation
- Main IPC: C12N9/00
- IPC: C12N9/00
Abstract:
The present invention is directed to a mutant thermostable ligase having substantially higher fidelity than either T4 ligase or Thermus thermophilus ligase. The ligase of the present invention is a mutant of a wild-type thermostable ligase having a histidine adjacent a KXDG motif, where the mutant thermostable ligase has a mutation in its amino sequence where the histidine adjacent the KXDG motif in the wild-type thermostable ligase is replaced with an arginine, and wherein X is any amino acid. The DNA molecule encoding this enzyme as well as expression systems and host cells containing it are also disclosed. The thermostable ligase of the present invention is useful in carrying out a ligase detection reaction process and a ligase chain reaction process.
Public/Granted literature
- US20140106431A1 HIGH FIDELITY THERMOSTABLE LIGASE AND USES THEREOF Public/Granted day:2014-04-17
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