A positioning module of calculating a coordinate of a touch medium is disclosed in the present invention. The positioning module includes at least one image detecting unit, a first light source, a second light source and a processor. The image detecting unit includes a lateral side and an upper side. The image detecting unit captures an image reflected from a reflection component. The first light source is disposed on the lateral side and outputs a first beam. The second light source is disposed on the upper side and outputs a second beam. The processor is electrically connected to the image detecting unit. The processor determines a touch status of the touch medium according to a first image generated by the first beam, and further determines a touch coordinate of the touch medium according to a second image generated by the second beam.