A transmission method for a display device is provided. The display device includes a one-to-many timing controller and a plurality of source drivers. The transmission method includes determining whether the plurality of source drivers being operated in different operational modes are switched on or off according to a command signal, to receive a display information of the one-to-many timing controller. The display information comprises at least a first control triggering signal, a first control signal, a first display information triggering signal, a first display signal, a second display information triggering signal and a second display signal, and the command signal is an internal setting signal or an external setting signal.