发明授权
- 专利标题: Method for detection of target nucleic acid
- 专利标题(中): 检测靶核酸的方法
-
申请号: US13497000申请日: 2010-10-26
-
公开(公告)号: US09175339B2公开(公告)日: 2015-11-03
- 发明人: Mitsuo Kawase , Toshikazu Hirota , Kousuke Niwa
- 申请人: Mitsuo Kawase , Toshikazu Hirota , Kousuke Niwa
- 申请人地址: JP Aichi Prefecture
- 专利权人: NGK Insulators, Ltd.
- 当前专利权人: NGK Insulators, Ltd.
- 当前专利权人地址: JP Aichi Prefecture
- 代理机构: Cermak Nakajima & McGowan LLP
- 代理商 Tomoko Nakajima
- 优先权: JP2009-249122 20091029
- 国际申请: PCT/JP2010/068964 WO 20101026
- 国际公布: WO2011/052586 WO 20110505
- 主分类号: C12Q1/68
- IPC分类号: C12Q1/68
摘要:
An object of the disclosure of the present specification is to provide a method for detection of a target nucleic acid which allows construction of an effective detection system of a target nucleic acid. For this purpose, in the disclosure of the present specification, a first primer comprising an identification sequence complementary to a target sequence in a target nucleic acid and a tag addition sequence, and a second primer having a label are prepared. The first primer and the second primer are used for the target nucleic acid in a sample to amplify a chimeric DNA having a tag sequence and the label. The chimeric DNA is hybridized with a detection probe on a solid phase to obtain signal intensity information based on the label, and the target nucleic acid is detected based on the signal intensity information.
公开/授权文献
- US20120178648A1 METHOD FOR DETECTION OF TARGET NUCLEIC ACID 公开/授权日:2012-07-12
信息查询