发明授权
- 专利标题： Development of a marker foot and mouth disease virus vaccine candidate that is attenuated in the natural host
- 专利标题（中）： 开发在天然宿主中减毒的标志物口蹄疫病毒疫苗候选物
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申请号： US14280984申请日： 2014-05-19
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公开(公告)号： US09180179B1公开(公告)日： 2015-11-10
- 发明人: Aida E. Rieder , Luis L. Rodriguez , Jason R. Hollister , Sabena Uddowla
- 申请人： The United States of America, as represented by the Secretary of Agriculture
- 申请人地址： US DC Washington
- 专利权人： The United States of America, as represented by The Secretary of Agriculture
- 当前专利权人： The United States of America, as represented by The Secretary of Agriculture
- 当前专利权人地址： US DC Washington
- 代理商 John D. Fado; Evelyn M. Rabin
- 主分类号： G01N33/569
- IPC分类号： G01N33/569 ; A61K39/135 ; C12N7/00
摘要：
We have generated novel molecularly marked FMDV A24LL3DYR and A24LL3BPVKV3DYR vaccine candidates. The mutant viruses contain a deletion of the leader coding region (LL) rendering the virus attenuated in vivo and negative antigenic markers introduced in one or both of the viral non-structural 3Dpol and 3B proteins. The vaccine platform includes unique restriction endonuclease sites for easy swapping of capsid proteins for different FMDV subtypes and serotypes. The mutant viruses produced no signs of FMD and no shedding of virulent virus in cattle. No clinical signs of disease or fever were observed and no transmission to in-contact animals was detected in pigs inoculated with live A24LL3DYR. Cattle immunized with chemically inactivated vaccine candidates showed an efficacy comparable to a polyvalent commercial FMDV vaccine. These vaccine candidates used in conjunction with a cELISA provide a suitable target for DIVA companion tests.
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