Invention Grant
- Patent Title: Ligase-assisted nucleic acid circularization and amplification
- Patent Title (中): 连接酶辅助核酸环化和扩增
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Application No.: US13952040Application Date: 2013-07-26
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Publication No.: US09217167B2Publication Date: 2015-12-22
- Inventor: Ryan Charles Heller , John Richard Nelson , Erik Leeming Kvam
- Applicant: General Electric Company
- Applicant Address: US NY Niskayuna
- Assignee: General Electric Company
- Current Assignee: General Electric Company
- Current Assignee Address: US NY Niskayuna
- Agent Jenifer E. Haeckl
- Main IPC: C12P19/34
- IPC: C12P19/34 ; C12Q1/68
Abstract:
Provided herein are methods for generation and amplification of a single-stranded DNA circle in a single reaction vessel from a linear DNA without any intervening purification steps. The single-stranded DNA circle is generated via a template-independent single-stranded DNA ligation. Whole-genome amplification of circulating nucleic acids extracted from blood is provided. Kits for performing the disclosed methods are also provided.
Public/Granted literature
- US20150031086A1 LIGASE-ASSISTED NUCLEIC ACID CIRCULARIZATION AND AMPLIFICATION Public/Granted day:2015-01-29
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