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US09249402B2 Process for the continuous production of cellulases by a filamentous fungus using a carbon substrate obtained from an acid pretreatment 有权
使用从酸预处理获得的碳底物通过丝状真菌连续生产纤维素酶的方法

Process for the continuous production of cellulases by a filamentous fungus using a carbon substrate obtained from an acid pretreatment
Abstract:
A process for the production of cellulases and hemicellulases using a strain of a filamentous fungus in a stirred and aerated bioreactor, in at least two phases, a phase a) for growth of said strain in the presence of at least one carbonaceous growth substrate in a closed reactor, a phase b) for the continuous production of cellulases, in which at least one carbonaceous inducer substrate is supplied at a supply rate which is constant over a period of at least more than 200 h, said carbonaceous substrate being at least one aqueous hemicellulosic hydrolysate solution obtained from an acid pre-treatment of a lignocellulosic substrate, said aqueous hemicellulosic hydrolysate solution not undergoing prior sterilization and not undergoing pH rectification, being operated at a dilution rate in the range 0.001 to 0.02 h−1.
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