Invention Grant
- Patent Title: Process for the continuous production of cellulases by a filamentous fungus using a carbon substrate obtained from an acid pretreatment
- Patent Title (中): 使用从酸预处理获得的碳底物通过丝状真菌连续生产纤维素酶的方法
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Application No.: US14350827Application Date: 2012-09-26
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Publication No.: US09249402B2Publication Date: 2016-02-02
- Inventor: Fadhel Ben Chaabane , Bernard Chaussepied
- Applicant: IFP ENERGIES NOUVELLES
- Applicant Address: FR Rueil-Malmaison
- Assignee: IFP Energies Nouvelles
- Current Assignee: IFP Energies Nouvelles
- Current Assignee Address: FR Rueil-Malmaison
- Agency: Millen White Zelano and Branigan, PC
- Agent Csaba Henter; Anthony Zelano
- Priority: FR1103149 20111014
- International Application: PCT/FR2012/000381 WO 20120926
- International Announcement: WO2013/054005 WO 20130418
- Main IPC: C12N9/42
- IPC: C12N9/42 ; C12P21/00 ; C12N1/14 ; C12N1/38 ; C12N9/18

Abstract:
A process for the production of cellulases and hemicellulases using a strain of a filamentous fungus in a stirred and aerated bioreactor, in at least two phases, a phase a) for growth of said strain in the presence of at least one carbonaceous growth substrate in a closed reactor, a phase b) for the continuous production of cellulases, in which at least one carbonaceous inducer substrate is supplied at a supply rate which is constant over a period of at least more than 200 h, said carbonaceous substrate being at least one aqueous hemicellulosic hydrolysate solution obtained from an acid pre-treatment of a lignocellulosic substrate, said aqueous hemicellulosic hydrolysate solution not undergoing prior sterilization and not undergoing pH rectification, being operated at a dilution rate in the range 0.001 to 0.02 h−1.
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