A display substrate for a display device includes: a substrate which includes a light blocking region defining a plurality of pixel areas disposed in a matrix, each pixel area having a length extending in a second direction, and a width extending in a first direction; a color filter overlapping a portion of the each pixel area of the plurality of pixel areas; and an alignment layer disposed on the color filter. the color filter includes a first edge parallel to the second direction, and a second edge forming a predetermined angle with the first edge. The second edge is substantially parallel to an alignment direction of the alignment layer.