Aspects of a method and system for an operating system (OS) virtualization-aware network interface card (NIC) are provided. A NIC may provide direct I/O capabilities for each of a plurality of concurrent guest operating systems (GOSs) in a host system. The NIC may comprise a GOS queue for each of the GOSs, where each GOS queue may comprise a transmit (TX) queue, a receive (RX) queue, and an event queue. The NIC may communicate data with a GOS via a corresponding TX queue and RX queue. The NIC may notify a GOS of events such as down link, up link, packet transmission, and packet reception via the corresponding event queue. The NIC may also support unicast, broadcast, and/or multicast communication between GOSs. The NIC may also validate a buffered address when the address corresponds to one of the GOSs operating in the host system.