A patterned color conversion film and a display device using the same are disclosed. The patterned color conversion film of the present invention comprises: a separator with plural openings; and plural pixel units disposed in the openings respectively, each pixel unit respectively comprising: a medium and scattering particles dispersed therein. Herein, at least one of the plural pixel units comprises quantum dots having the scattering particles sized of between 0.05 and 1 μm when a volume concentration of the quantum dots is in a range more than or equal to 5% and less than or equal to 80%, or having the scattering particles sized of between 0.2 and 2 μm when the volume concentration of the quantum dots is in a range less than 5% and more than or equal to 0%.