发明授权
US09273312B2 SiRNA sequence-independent modification formats for reducing off-target phenotypic effects in RNAi, and stabilized forms thereof
有权
用于减少RNAi中的靶外表型效应的siRNA序列非依赖性修饰形式及其稳定形式
- 专利标题： SiRNA sequence-independent modification formats for reducing off-target phenotypic effects in RNAi, and stabilized forms thereof
- 专利标题（中）： 用于减少RNAi中的靶外表型效应的siRNA序列非依赖性修饰形式及其稳定形式
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申请号： US13975136申请日： 2013-08-23
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公开(公告)号： US09273312B2公开(公告)日： 2016-03-01
- 发明人: Nitin Puri , Irudaya Charles , Susan Magdaleno , Alexander Vlassov , Christopher Burnett
- 申请人： Applied Biosystems, LLC
- 申请人地址： US CA Carlsbad
- 专利权人： Applied Biosystems, LLC
- 当前专利权人： Applied Biosystems, LLC
- 当前专利权人地址： US CA Carlsbad
- 代理商 Gloria L. Norberg
- 主分类号： C12N15/113
- IPC分类号： C12N15/113 ; C12N15/11
摘要：
Modification formats having modified nucleotides are provided for siRNA. Short interfering RNA having modification formats and modified nucleotides provided herein reduce off-target effects in RNA interference of endogenous genes. Further modification formatted siRNAs are demonstrated to be stabilized to nuclease-rich environments. Unexpectedly, increasing or maintaining strand bias, while necessary to maintain potency for endogenous RNA interference, is not sufficient for reducing off-target effects in cell biology assays.
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