发明授权
US09279112B2 Cellulase enzymes having a modified linker and reduced lignin binding
有权
具有改性接头和减少木质素结合的纤维素酶
- 专利标题: Cellulase enzymes having a modified linker and reduced lignin binding
- 专利标题(中): 具有改性接头和减少木质素结合的纤维素酶
-
申请号: US12713516申请日: 2010-02-26
-
公开(公告)号: US09279112B2公开(公告)日: 2016-03-08
- 发明人: Brian R. Scott , Patrick St-Pierre , James Lavigne , Nabil Masri , Theresa C. White , John J. Tomashek
- 申请人: Brian R. Scott , Patrick St-Pierre , James Lavigne , Nabil Masri , Theresa C. White , John J. Tomashek
- 申请人地址: CA Ontario
- 专利权人: IOGEN ENERGY CORPORATION
- 当前专利权人: IOGEN ENERGY CORPORATION
- 当前专利权人地址: CA Ontario
- 代理机构: Fitzpatrick, Cella, Harper & Scinto
- 主分类号: C12N9/42
- IPC分类号: C12N9/42 ; C12P19/14
摘要:
Provided are modified cellulase enzymes exhibiting increase cellulose-hydrolyzing activity in the presence of lignin and/or reduced binding to lignin comprising modified linker peptides comprising one or more amino acid substitutions, insertions, or deletions that result in (a) a decrease in the calculated isoelectric point of the linker peptide and/or (b) an increase in the ratio of threonine:serine in the linker peptide relative to a parental linker peptide from which said modified linker peptide is derived. Also provided are genetic constructs comprising nucleic acid sequences encoding for modified cellulase enzymes, methods for the production of the modified cellulase enzymes from host strains and a process for hydrolyzing cellulose with the modified cellulases in the presence of lignin.
公开/授权文献
- US20100221778A1 NOVEL LIGNIN-RESISTANT CELLULASE ENZYMES 公开/授权日:2010-09-02
信息查询
IPC分类: