An exposure mechanism of an optical touch system, which includes a plurality of image sensors and a plurality of active light sources each irradiating corresponding to the associated image sensor, includes: capturing image frames using the image sensors with a sampling cycle to allow each of the image sensors to capture a bright image, wherein the sampling cycle includes a plurality of working modes and in each of the working modes at least one of the image sensors captures the bright image in a sampling interval; simultaneously capturing a dark image using all the image sensors in a denoising sampling interval; and calculating a differential image between the bright image and the dark image captured by each image sensor.