A method for separating and estimating multiple motion parameters in an X-ray angiogram image. The method includes: determining a cardiac motion signal cycle and a variation frame sequence of translational motion according to an angiogram image sequence, tracing structure feature points of vessels in the angiogram image sequence whereby obtaining a motion sequence, processing the motion sequence via multivariable optimization and Fourier frequency-domain filtering, separating an optimum translational motion curve, a cardiac motion curve, a respiratory motion curve and a high-frequency motion curve according to the variation frame sequence of translational motion, a cycle of the cardiac motion signal, a range of a respiratory motion signal cycle, and a range of a high-frequency motion signal cycle.