An apparatus for generating pulsatile flows includes a liquid vessel capable of containing a liquid, a plurality of revolving mechanisms associated with each other, and a microchannel supplied with a liquid from the liquid vessel. As the plurality of revolving mechanisms rotate, a periodically changing pressure difference occurs between the liquid vessel and the microchannel, thereby implementing a pulsatile flow having a wave functional form in the microchannel. By applying the hydraulic head difference and controlling revolution of the revolving mechanisms based on Fourier cosine series, a minute and precise pulsatile flow of a wave functional form may be implemented by means of simple configuration and fabrication, which may not easily obtained by a conventional pump.