Systems and methods described herein facilitate determining desktop readiness using interactive measures. A host is in communication with a server and the host includes a virtual desktop and a virtual desktop agent. The virtual desktop agent is configured to perform one or more injecting events via one or more monitoring agents, wherein each of the injecting events is a simulated input device event. The desktop agent is further configured to receive, via a display module, a response to the injecting event(s), wherein the response is a display update causing pixel color values for the display module to alter. The desktop agent is also configured to identify, via the monitoring agent(s), whether the response to the injecting event(s) is an expected response. The desktop agent is also configured to determine, via the monitoring agent(s), a readiness of the virtual desktop based on the expected response.