An assembly structure for a server hard disk back panel includes a server chassis; a plurality of hard disk frames disposed inside the server chassis; and a back panel disposed between the server chassis and the hard disk frame. A hook is disposed, corresponding to the back panel, on a side edge of each of the two side portions of each hard disk frame. The hooks of any two adjacent hard disk frames are disposed adjacent to each other correspondingly. The back panel includes a plurality of hook holes, and a respective one of the hook holes is corresponding to each two adjacent hooks. Each of the hook holes includes two contact edges corresponding to each other at two sides and includes a hook edge between the two contact edges.