A cleaning equipment of sample bottle for chromatography analysis comprising: a base; a support frame vertically fixed to the base; a horizontal column having two ends, wherein one of the ends is disposed at the support frame through an adjustment knob; a rotary drive device fixed to the other end of the horizontal columns; a rotation disk which can rotate through driving of the rotary drive device, and a side edge of the rotation disk provides with multiple placing holes for receiving and fixing sample bottles; a cleaning agent recycling tank disposed below the rotation disk, wherein a side wall of it provides with an ejector pipe connected with a water pump; and a control unit for controlling on/off and rotation speed of the rotary drive device, and on/off of the water pump. The invention also provides a cleaning method of sample bottle for chromatography analysis.