The present disclosure provides a light source apparatus, the apparatus including a light source configured to irradiate an excitation light, an optical system configured to form the excitation light, and a phosphor wheel configured to separate a plurality of colors based on the formed excitation light, wherein the phosphor wheel includes a first separation portion of light-transmitting material assembled as a separate element penetrable by a blue light of the excitation light, and integrally formed second to fourth separation portions having phosphor layer configured to reflect red, green and yellow of the excitation light.