An over-voltage protection circuit is applied to a switching voltage converting circuit. The switching voltage converting circuit manipulates an upper bridge power switch in the circuit, so as to convert an input voltage into an output voltage by an inductor. A channel of the upper bridge power switch and the inductor are coupled to a phase end. The over-voltage protection circuit includes: a comparator, coupled to the switching voltage converting circuit, wherein when a voltage of the phase end is higher than a voltage limiting threshold, an output end of the comparator outputs a first voltage level; and a pulse width detection unit, coupled to the output end of the comparator, wherein when the output end of the comparator remains the first voltage level for a time period longer than a protection period, the pulse width detection unit outputs an over-voltage protection activation signal.