This invention provides a means for measuring a cholesterol content of a chicken egg by a non-destructive means. This invention relates to a method for measuring a cholesterol content of a chicken egg comprising: a step of light irradiation of irradiating the chicken egg with light in the visible to near-infrared wavelength range; a step of light detection of detecting light radiated from the chicken egg caused by that the light irradiated in the step of light irradiation is transmitted through the chicken egg or reflected inside the chicken egg; a step of spectrum acquisition of acquiring a near-infrared spectrum of the light detected in the step of light detection; and a step of cholesterol content determination of determining a cholesterol content of the chicken egg on the basis of the near-infrared spectrum acquired in the step of spectrum acquisition.