Embodiments of imaging systems and methods of autofocusing are disclosed, for example, using a folded optics configuration. One system includes at least one camera configured to capture a target image scene, including an image sensor comprising an array of sensor elements, a primary light folding surface configured to direct a portion of received light in a first direction, and an optical element having a secondary light folding surface directing light in a second direction. The system can also include a lens assembly having at least one stationary lens positioned between the secondary light folding surface and the image sensor, the at least one stationary lens having a first surface mechanically coupled to the optical element and a second surface mechanically coupled to the image sensor, and at least one movable lens positioned between the primary light folding surface and the optical element.