A method for removal of a hydrate plug (7) in a tube (92) in connection with a petroleum well (9) is described. A hydrate plug remover (2) is included having a collecting container (21). The method includes the steps of positioning the hydrate plug remover (2) in a sluice (5); filling the sluice (5) with a hydrate dissolving fluid; filling the collecting container (21) provided with a valve (3) in the lower end portion (20) of the collecting container (21) with hydrate dissolving fluid through the valve (3); pressure testing the sluice (5); displacing the hydrate plug remover (2) from the hydrate plug (7) and letting the hydrate dissolving fluid flow towards the hydrate plug (7) through a circulation gate (22) in the upper end portion (29) of the collecting container (21); leading loosened hydrate from the hydrate plug (7) into the collecting container (21) via the valve (3); and displacing the hydrate plug remover (2) to the sluice (5) where the collecting container (21) is emptied of hydrate through the circulation gate (22) while hydrate dissolving fluid flows into the collection container (21) through the valve (3). A hydrate plug remover for performing the method is also described.