发明授权
- 专利标题: Poly(vinyl benzoate) nanoparticles for molecular delivery
- 专利标题(中): 用于分子递送的聚(乙烯基苯甲酸酯)纳米颗粒
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申请号: US13897868申请日: 2013-05-20
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公开(公告)号: US09433581B2公开(公告)日: 2016-09-06
- 发明人: Edward Turos , Raphael Labruere , Ryan Scott Cormier , Renaud Sicard
- 申请人: Edward Turos , Raphael Labruere , Ryan Scott Cormier , Renaud Sicard
- 申请人地址: US FL Tampa
- 专利权人: University of South Florida
- 当前专利权人: University of South Florida
- 当前专利权人地址: US FL Tampa
- 代理机构: Smith & Hopen, P.A.
- 代理商 Michele L. Lawson
- 主分类号: A61K9/14
- IPC分类号: A61K9/14 ; A61K9/00 ; A61K9/10 ; A61K9/51 ; A61K47/10 ; A61K47/48 ; A61K49/00
摘要:
The present invention comprises poly(vinyl benzoate) nanoparticle suspensions as molecular carriers. These nanoparticles can be formed by nanoprecipitation of poly(vinyl benzoate) in water using Pluronic F68 as surfactant, to create spherical nanostructures measuring about 200-250 nm in diameter which are stable in phosphate buffer and blood serum, and only slowly degrade in the presence of esterases. Kinetics experiments in phosphate buffer indicate that 78% of the coumarin-6 was encapsulated within the polymer matrix of the nanoparticle, and the residual 22% of coumarin-6 was surface-bound and quickly released. The nanoparticles are non-toxic in vitro towards human epithelial cells (IC50>1000 μg/mL) and primary bovine primary aortic endothelial cells (IC50>500 μg/mL), and exert non-observable bactericidal activity against a selection of representative test microbes (MIC>250 μg/mL). Poly(vinyl benzoate) nanoparticles are suitable carriers for molecular delivery of lipophilic small molecules such as drugs pharmaceutical and imaging agents.
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