Invention Grant
- Patent Title: Polymer particles, nucleic acid polymer particles and methods of making and using the same
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Application No.: US14640009Application Date: 2015-03-05
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Publication No.: US09434805B2Publication Date: 2016-09-06
- Inventor: David Light , Barnett B. Rosenblum
- Applicant: LIFE TECHNOLOGIES CORPORATION
- Applicant Address: US CA Carlsbad
- Assignee: Life Technologies Corporation
- Current Assignee: Life Technologies Corporation
- Current Assignee Address: US CA Carlsbad
- Main IPC: C08F251/00
- IPC: C08F251/00 ; C08F2/32 ; C08F220/56 ; C12Q1/68

Abstract:
The disclosure relates to methods of making polymer particles, said methods including the steps of: making an aqueous gel reaction mixture; forming an emulsion having dispersed aqueous phase micelles of gel reaction mixture in a continuous phase; adding an initiator oil comprising at least one polymerization initiator to the continuous phase; and performing a polymerization reaction in the micelles. Further, the initiator oil is present in a volume % relative to a volume of the aqueous gel reaction mixture of between about 1 vol % to about 20 vol %. The disclosure also relates to methods of making nucleic acid polymer particles having the same method steps and wherein the aqueous gel reaction mixture includes a nucleic acid fragment, such as a primer.
Public/Granted literature
- US20150175734A1 POLYMER PARTICLES, NUCLEIC ACID POLYMER PARTICLES AND METHODS OF MAKING AND USING THE SAME Public/Granted day:2015-06-25
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