Invention Grant
- Patent Title: Water-soluble polypeptides comprised of repeat modules, method for preparing the same and method for a target-specific polypeptide and analysis of biological activity thereof
- Patent Title (中): 由重复模块组成的水溶性多肽，其制备方法和靶特异性多肽的方法及其生物活性的分析
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Application No.: US14005536Application Date: 2012-03-22
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Publication No.: US09441017B2Publication Date: 2016-09-13
- Inventor: Hak Sung Kim , Dong Sup Kim , Sang Chul Lee , Byung Chul Lee , Ji Eun Han , Joong Jae Lee , Keun Wan Park , Seung Pyo Hong
- Applicant: Hak Sung Kim , Dong Sup Kim , Sang Chul Lee , Byung Chul Lee , Ji Eun Han , Joong Jae Lee , Keun Wan Park , Seung Pyo Hong
- Applicant Address: KR Daejeon
- Assignee: KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY
- Current Assignee: KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY
- Current Assignee Address: KR Daejeon
- Agency: Gavrilovich, Dodd & Lindsey LLP
- Agent Joseph R. Baker, Jr.
- Priority: KR10-2011-0025446 20110322
- International Application: PCT/KR2012/002094 WO 20120322
- International Announcement: WO2012/128580 WO 20120927
- Main IPC: C07K14/195
- IPC: C07K14/195 ; C07K14/705 ; G01N33/50 ; G06F19/16 ; C12Q1/68 ; C07K14/46 ; C12N15/70
Abstract:
The present invention relates to a soluble polypeptide comprised of repeat modules. More particularly, the present invention relates to a soluble fusion polypeptide of the N-terminal domain of internalin and LRR (Leucine rich repeat) family protein, a method for preparing the polypeptide, a vector comprising a nucleic acid sequence encoding the polypeptide, a host cell comprising the vector, a method for producing a solubility and folding-improved fusion polypeptide by expressing the vector in the host cell, and a method for improving the solubility and folding of the fusion polypeptide. Further, the present invention relates to a method for preparing the polypeptide bound with a specific target and analyzing the efficacy of the soluble polypeptide.
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