Provided is a method of transmitting control information using a physical uplink shared channel (PUSCH) region in a system employing a multi-input multi-output (MIMO) antenna system in which transmission is performed through a plurality of layers. A method of transmitting a channel quality indicator (CQI)/precoding matrix indicator (PMI) using a PUSCH region in a system employing a MIMO antenna system in which transmission is performed through a plurality of layers includes encoding the CQI/PMI using one channel encoder, and transmitting the encoded CQI/PMI using some or all of the layers. Accordingly, it is possible to transmit uplink control information through a PUSCH region using a plurality of layers.