A method of compensating a Mura defect of a display apparatus, which includes a display area divided into an upper area and a lower area, includes calculating a sharp grayscale correction value of a predetermined sample grayscale displayed on the display apparatus, where the sharp grayscale correction value is configured to compensate a sharp horizontal Mura between the upper and lower areas, displaying a corrected sample grayscale on the display apparatus based on the predetermined sample grayscale and the sharp grayscale correction value, sensing the corrected sample grayscale displayed on each of a plurality of sample areas defined on the display area based on a Mura type, calculating an intensity profile of the sample grayscale and a target intensity profile configured to compensate the intensity profile of the sample grayscale, calculating a grayscale correction value of the sample area using the intensity profile and the target intensity profile.