A gaming system including a cascading symbol game which utilizes a plurality of interacting symbols. In certain embodiments, the cascading symbol game utilizes a plurality of symbol display position matrices or grids. Each symbol display position matrix includes a plurality of symbol display positions. In operation, the gaming system generates and displays a symbol at each symbol display position of each employed symbol display position matrix, wherein the gaming system utilizes a separate set or pool of available symbols for each employed symbol display position matrix. Upon an occurrence of an interacting symbol triggering event, such as the shifting of one or more symbols between symbol display position matrices, the gaming system causes one or more symbols to interact to provide one or more benefits to a player.