A method and apparatus are provided for segmenting an object in an image. The method includes obtaining a first image including the object; receiving an input signal including information about a predetermined position in the first image; selecting at least one pixel included in the first image, based on the position information; generating a second image by dividing the first image into several areas, using the selected at least one pixel; and segmenting the object in the first image by using the first image and the second image.