Provided is an optical imaging system capable of increasing diagnosis reliability and preciseness, and efficiently observing a target. The optical imaging system using multiple light sources according to an embodiment of the present invention includes a first light source generating a first light modulated with a first frequency, a second light source generating a second light modulated with a second frequency, a camera simultaneously detecting multiple lights output from an object after the first and second lights are illuminated on the object and outputting multiple image detecting signals, and an image processing unit processing the multiple image detecting signals to obtain a first image representing a shape of the object and a second image representing a desired target portion of the object.