A method and an apparatus for operating a User Interface (UI) of a conference call terminal are provided. The method includes operating a first camera and a second camera when a conference call is initiated, outputting an output image of a conference call screen comprising a first image and a second image collected from the first camera and the second camera, the first image and the second image being arranged not to overlap, and distinguishing a speaking person's image from another person's image, when the speaking person's image is detected in the output image of the conference call screen.