The present disclosure provides a detection device for lithium-ion battery, which comprises an insulative housing having a receiving chamber; an insulative separator positioned between the positive electrode sheet and the negative electrode sheet when the positive electrode sheet and the negative electrode sheet are received in the receiving chamber; a positive electrode sheet conductive fastener passing through the insulative housing and fixedly connected to a positive electrode current collector at a positive electrode current collector non-film-coating region; a negative electrode sheet conductive fastener passing through the insulative housing and fixedly connected to a negative electrode current collector at a negative electrode current collector non-film-coating region; an insulative cover engaged with the insulative housing and the insulative separator; a positive electrode region detection hole communicated to the positive electrode sheet gas region; and a negative electrode region detection hole communicated to the negative electrode sheet gas region.