The present invention provides an array substrate, a driving method of the array substrate and a display device comprising the array substrate. The array substrate comprises a display region and a non-display region, multiple groups of source lines and a plurality of gate lines are arranged in the display region, the multiple groups of source lines and the plurality of gate lines are intersected with each other to divide the display region into a plurality of pixel regions, and each pixel region corresponds to a group of source lines, wherein a first electrode and a second electrode are arranged in each pixel region, and the first electrode and the second electrode are adjacent and electrically isolated from each other.