Systems and methods for multi-focus imaging are disclosed. An imaging system may suggest multiple regions of interest to a user based on a preview mode of a scene of interest. The user may select a plurality of the suggested regions of interest for a multi-focus image. The imaging system calculates the autofocus parameters for each of the multiple regions of interest and captures single images of each of the regions of interest when the shutter button of the camera is pressed. The imaging system then generates a multi-focus image from the multiple images.