Invention Grant
- Patent Title: Polymerase idling method for single molecule DNA sequencing
- Patent Title (中): 用于单分子DNA测序的聚合酶空转方法
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Application No.: US14538632Application Date: 2014-11-11
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Publication No.: US09546398B2Publication Date: 2017-01-17
- Inventor: Brian Jon Peter , John Mannion , Joel Myerson
- Applicant: Agilent Technologies, Inc.
- Applicant Address: US CA Santa Clara
- Assignee: Agilent Technologies, Inc.
- Current Assignee: Agilent Technologies, Inc.
- Current Assignee Address: US CA Santa Clara
- Main IPC: C12Q1/68
- IPC: C12Q1/68

Abstract:
A method for sequencing a nucleic acid is provided. In certain embodiments the method comprises obtaining a duplex comprising a nucleic acid and a primer, wherein the primer has a nuclease resistant 3′ end, combining the duplex with a chain terminator nucleotide and a proof-reading polymerase to produce a reaction in which the polymerase idles on the added chain terminator nucleotide, identifying the chain terminator nucleotide added to the end of the primer; and adding a nuclease-resistant nucleotide to the end of the primer after the polymerase has idled on and removed the added chain terminator nucleotide, thereby producing a duplex comprising the template and an extended primer that has a nuclease resistant 3′ end.
Public/Granted literature
- US20150132756A1 POLYMERASE IDLING METHOD FOR SINGLE MOLECULE DNA SEQUENCING Public/Granted day:2015-05-14
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