An electro-wetting display panel is provided. The electro-wetting display panel comprises a first transparent substrate, a second transparent substrate, and pixel units. The pixel units comprise first electrodes, a first hydrophobic layer, a plurality of first barriers, a second electrode, a polarized liquid and a plurality of first non-polarized liquids. The hysteresis phenomenon of the non-polarized liquids can be prevented by using the recess areas on the hydrophobic layer.