Invention Grant
US09567592B2 Method for expression of small antiviral RNA molecules with reduced cytotoxicity within a cell
有权
用于表达细胞内细胞毒性降低的小型抗病毒RNA分子的方法
- Patent Title: Method for expression of small antiviral RNA molecules with reduced cytotoxicity within a cell
- Patent Title (中): 用于表达细胞内细胞毒性降低的小型抗病毒RNA分子的方法
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Application No.: US14695462Application Date: 2015-04-24
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Publication No.: US09567592B2Publication Date: 2017-02-14
- Inventor: Carlos Lois-Caballe , David Baltimore , Xiao-Feng Qin , Irvin S. Y. Chen , Dong Sung An
- Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY , The Regents of the University of California
- Applicant Address: US CA Pasadena US CA Oakland
- Assignee: California Institute of Technology,The Regents of the University of California
- Current Assignee: California Institute of Technology,The Regents of the University of California
- Current Assignee Address: US CA Pasadena US CA Oakland
- Agency: Knobbe Martens Olson & Bear LLP
- Main IPC: C12N15/113
- IPC: C12N15/113 ; C12N15/867 ; A61P31/18 ; C12N15/63 ; C12N15/11 ; C12N15/86 ; A61K48/00

Abstract:
In one aspect, the invention provides methods and compositions for the expression of small RNA molecules within a cell using a retroviral vector (FIG. 1A). Small interfering RNA (siRNA) can be expressed using the methods of the invention within a cell. In a further aspect, the invention provides methods for producing siRNA encoding lentivirus where the siRNA activity may interfere with the lentiviral life cycle. In yet a further aspect, the invention provides methods for expression of a small RNA molecule within a cell, such as an siRNA capable of downregulating CCR5, wherein expression of the small RNA molecule is relatively non-cytotoxic to the cell. The invention also includes small RNA molecules, such as an siRNA capable of downregulating CCR5, that are relatively non-cytotoxic to cells.
Public/Granted literature
- US20150218564A1 METHOD FOR EXPRESSION OF SMALL ANTIVIRAL RNA MOLECULES WITH REDUCED CYTOTOXICITY WITHIN A CELL Public/Granted day:2015-08-06
Information query
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