A method is provided for transmitting, by a user equipment (UE), a demodulation reference signal (DMRS) for a physical uplink shared channel (PUSCH) in a wireless communication system. First, second, and third DMRS sequences associated with first, second, and third layers respectively are generated. First, second, and third cyclic shifts are allocated to the first, second, and third DMRS sequences respectively, The first, second, and third DMRS sequences are transmitted to a base station. The first, second, and third cyclic shifts are respectively determined based on first, second and third cyclic shift values, which are indicated by a cyclic shift field in downlink control information (DCI) received through a physical downlink control channel (PDCCH). The first and second cyclic shift values are separated by a maximum separation value corresponding to a total number of cyclic shifts and being determined for transmission of DMRS signal in the system using four layers.