The disclosure relates to an array substrate and a manufacturing method thereof, and a display panel. The array substrate includes a plurality of data lines and a plurality of first gate lines, a plurality of first pixel units and a plurality of second pixel units; a plurality of second gate lines; a first TFT and a second TFT, where a first electrode of the first TFT is disposed at one side of the second gate line, the gate and the second electrode of the first TFT is disposed at the other side of the second gate line, the gate, the first electrode, the second electrode and the active layer of each second TFT are disposed at the same side of the second gate line, where the first electrodes of the first TFT and the second TFT are electrically connected to the date lines respectively.